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Transmittance measurements

Figure 4: Collection of scattered light by an integrating sphere. I

= incident light, I

scattered light.

In order to perform useful measurements on scattering samples, it is necessary to collect a

high proportion of the scattered radiation. The integrating sphere is a highly efficient

collector of scattered radiation. Because of its design, the Diffuse Reflectance accessory

overcomes many of the problems associated with measuring turbid or scattering samples,

which include sloping baseline, poor signal-to-noise ratio and high background

absorbance.

Whilst the DRA should be used when measuring the transmission of opaque or diffusing

solid samples, the accessory may also be used when measuring turbid liquids.

Because the cuvette is located so as to be part of the wall of the sphere, a greater

proportion of the scattered radiation transmitted by the sample is collected by the sphere,

as shown in Figure 3 above

With the PTFE reference disk in position, a sample is placed in the cuvette, and light is

passed through the sample.

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Mantis 5000 Especificaciones Pagina 6