Mantis 5000 Especificaciones descargar pdf (Pagina 67)

Transmission Measurements (Small Spot Kit)

At the transmission port location, the DRA small spot kit can be used to measure a small

test sample, or to measure a small portion of a larger sample. Prior to entering this

procedure, the small spot kit should be installed and aligned. Mirror M3 must always be

installed at the "T" position for small spot kit measurements.

1. Install the transmission lens, marked “T”, into the small spot kit lens holder. The

arrow marked on the lens should be pointing towards the sphere.

2. Darken the room and drive the instrument to 500 nm

3. If the intended sample size is smaller than the transmission port opening, secure a

mask over the transmission port. The mask should be attached to the transmission port or

holder, and it must remain in place for both the baseline and sample scans. If the sample

is larger than the port size, place a piece of translucent paper into the clips of the

transmission sample holder and install the holder at the transmission port.

4. Adjust the position of the small spot kit lens assembly on its dovetail rail until the

desired spot size is obtained at the transmission port. The spot should be adjusted to fit

just within the sample surface. In general, the transmission spot size will be smallest

when the lens is moved away from the sphere. To optimize the signal reaching the sphere

detectors, the iris should remain fully open for small spot kit transmission scans.

Figure 51. The beam at the transmission port

5. Collect a Baseline scan

, following the procedures specific to transmission

measurements. If the sample resides on a substrate, load an untreated substrate blank at

the transmission port for the baseline scan. If the transmission sample is suspended in

solution, load a blank cuvette at the transmission port for the baseline.

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Mantis 5000 Especificaciones Pagina 67