Mantis 5000 Especificaciones Pagina 61

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1. Collect a Baseline scan, following the procedures specific to transmission
measurements. If the sample resides on a substrate, load an untreated substrate blank at
the transmission port for the baseline scan. If the transmission sample is suspended in
solution, load a blank cuvette at the transmission port for the baseline.
2. Load the sample at the transmission port and replace the reflectance standard at the
reflectance port with a light trap. Do not change the configuration of the integrating
sphere in any other way.
3. Click the Setup button and check the parameters. Normally, the Setup parameters for
the application should match those used for the baseline scan. If using a previously
defined baseline correction, retrieve the correct baseline.
4. Click the Start button.
5. The value displayed by the instrument is the sample transmission. No data correction
is required.
Loading samples at the reference or reflectance ports
When taking a sample at either the sample or reflectance port requires the use of the
sample holder as shown below.
Figure 48. The sample is mounted at the reflectance or reference port using the sample
holder.
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